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Promega
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Becton Dickinson
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Lonza
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Image Search Results
Journal: Cell Proliferation
Article Title: DDIT 3 overexpression increases odontoblastic potential of human dental pulp cells
doi: 10.1111/cpr.12104
Figure Lengend Snippet: Immunofluorescent staining and endogenous DDIT3 expression in human dental pulp cells (HDPCs). (a) Immunofluorescent staining of DDIT3 in HDPCs without primary antibody (a‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (b) Immunofluorescent staining of DDIT3 in wild‐type HDPCs (b‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (c) Immunofluorescent staining showed increased nuclear accumulation of DDIT3 in TNFα‐challenged HDPCs (arrow, c‐1–3: DAPI staining, TRITC‐conjugated secondary antibody staining and merge). (d) mRNA expression of IL‐6, IL‐1β and DDIT3 in HDPCs after TNFα treatment. (e) Endogenous DDIT3 mRNA expression during odontoblastic differentiation detected by qRT‐PCR. (f) Endogenous DDIT3 protein expression during odontoblastic differentiation detected by Western blot. *Indicates a significant difference between two groups (P < 0.05). Scale bar = 50 μm.
Article Snippet: Secondary antibodies conjugated to
Techniques: Staining, Expressing, Quantitative RT-PCR, Western Blot